The Effect Of Different Brands Of Milk On Cholesterol-Induced Hyperglycemia In Adult Wistar Rats

Abstract:

This study is aimed at evaluating the effect of peak milk and three crown milk on hyperglycemic male Wistar rats. Twenty five (25) male adult Wistar rats were grouped into five (5) each containing five (5) rats. Groups I - IV were administered cholesterol serially diluted in distilled water and high fat diet ( 50% commercial grower feed, 30% groundnut meal and 20% groundnut oil) for a period of about 8 weeks. For group V which are the negative control group were fed with commercial growers feed without treatment. At the end of the 8 weeks, blood sample was collected from the tip of their tail for confirmation of the induction of hyperglycemia then followed by treatment. Group I were treated with three crown milk and group II were treated with peak milk while group III were treated with standard drug (glibenclamide) for a period of 2 weeks. The animals were sacrificed, both blood and tissues (liver) samples were collected for analysis. The result obtained from the blood analysis were subjected to statistical analysis at (p<0.05) using EZ analyser 3.0 statistical software tool. The result indicate that the peak milk and three crown have anti-hyperglycemic effect at (p <0.05) and both milks have an ameliorative effect on the serum levels of the biochemical parameters: liver enzymes; alkaline phosphatase, alanine aminotransferase and aspartate aminotransminase (p<0.05) and lipid profile; triglyceride, cholesterol, low density level lipoprotein and high density level lipoprotein (p<0.05). The two brands of milk have ameliorative effect on the histomorphology of liver. This effect was shown by the decreased vacuolisation caused by the induction of hyperglycemia.