Effect Of Prolactin In Sleep Deprived-Induced Stress In Adult Female Wistar Rats Treated With Ascorbic Acid And Tocopherol

Abstract:

Stress and its psychological manifestations are inherent in life and a major source of concern in the modern society. This study was designed to evaluate how stress affect the level of prolactin in sleep deprived induced stress in female wistar rats treated with ascorbic acid and tocopherol. Stress was induced by subjecting the female wistar rats to twenty (20) hours of sleep deprivation after which animals were allowed to rest for only four (4) hours for a period of seven (7) days using a modified platform technique. A total of twenty female wistar rats were randomly divided into four groups of five rats (n=5). Group I was the control which received 1ml/kg distilled water, group II was sleep deprived and untreated and received 1ml/kg distilled water, group III were treated with 100mg/kg vitamin C and group were treated with 100mg/kg vitamin E. After seven days the female wistar rats were sacrificed and blood sample was collected and centrifuged for biochemical analysis. The results show that there was an increase in prolactin level in the sleep deprived untreated group with that of control group. The sleep deprived group treated with vitamin C shows a significant (at p≤0.05) increase in prolactin level when compared with sleep deprived untreated group and control group. Whereas the sleep deprived group treated with vitamin E shows an insignificant (at p≤0.05) decrease in prolactin when compared with sleep deprived untreated group and control group. The finding of this study indicate that vitamin C increases the prolactin level in sleep deprived induced stress in female wistar rats whereas vitamin E does not have any significant effect in prolactin levels.