Effect Of Progesterone On Sleep Deprived-Induced Stress Female Wistar Rats Treated With Ascorbic Acid And Tocopherol

Abstract:

Stress is a major concern because it decreases the normal healthy function of the Human body. One of its effects is decreased Reproductive function therefore understanding of stress management is necessary for the maintenance of normal fetal life and normal healthy life in general. The aim of this study was to determine the effect of Progesterone on sleep deprived-induced stress Adult Female Wistar Rats when treated with Ascorbic acid and Tocopherol (Vitamin C and E). The sleep deprivation was conducted to induce stress. Rats were individually suspended in water for 20hours using platform technique. They were also allowed to rest for about 4hours each day for the next 7days. A total of twenty (20) Adult female Wistar rats were grouped into four groups with a total of five rats (n=5) in each group. Group I which is the control group received 1ml/kg of distilled water, Group II which is stress induced untreated received 1mg/kg of Distilled water, Group III was treated with 100mg/kg of Vitamin C and where sleep deprived (stress induced), and Group IV was treated with 100mg/kg of Vitamin E and were also sleep deprived. After seven (7) days the animals where sacrificed and blood samples were collected for biochemical analysis. A significant decrease (p<0.05) in progesterone level was observed in the stress induced and Vitamin E compared to the control group. A significant increase (p<0.05) between SD-Untreated compared to SD treated with Vitamin C was also observed. Also there was a significant decrease (p<0.05) found in the SD treated with Vitamin E when compared to Control and SD treated with Vitamin C. The results obtained and the findings indicate that Vitamin C increases the progesterone level in sleep deprived (stress induced) Adult female Wistar rats.