Effect Of Cortisol On Sleep Deprived-Induced Stress In Adult Female Wistar Rats Treated With Ascorbic Acid And Tocopherol

Abstract:

The female physiological system is a dynamic system; fluctuations in hormonal levels that arise due to physiologic function. Stress affects normal physiologic functions that could alter biosynthesis of hormones. Hence, the essence of this study was design to evaluate the effect of cortisol, prolactin, progesterone, estrogen, T3, T4 on sleep induce stress in adult female wistar rats treated with vitamin C and E. Using modified platform technique model, the animal was subjected to sleep induce stress by depriving them of sleep for 20hrs for a period of 7days. The animals were randomly divided into four groups of five (n=5) each, Group 1 (control) were administered 1 ml of distil water; group 2 were sleep deprived untreated and were administered 1ml of distil water; group 3 were sleep deprived treated with 100mg/kg of vitamin C and group 4 were sleep deprived treated with 100mg/kg of vitamin E. Sera from blood of euthanized animals was analysed for the assay of cortisol, prolactin, progesterone, estrogen, T3, T4. Findings from the study showed significant increase in level of cortisol when compared with control (433.60 ±12.60 vs 325.80 ±7.67). Groups treated with vitamin C and E showed a significant decrease (P<0.05) compared with untreated group. In conclusion, ascorbic acid and tocopherol reduces cortisol level in sleep induce stress of adult female wistar rat.